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Addgene

Progerin-S22D-CS-pLPC
(Plasmid #73810)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 7338
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Progerin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1845
  • Mutation
    Progerin with substitution of cysteine in CaaX-motif by serine (C to S) and S22D mutation
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer taggcgtgtacggtggga
  • 3′ sequencing primer acctgtaggtttggcaag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Progerin-S22D-CS-pLPC was a gift from Gerardo Ferbeyre (Addgene plasmid # 73810 ; http://n2t.net/addgene:73810 ; RRID:Addgene_73810)

  • For your References section:

    Permanent farnesylation of lamin A mutants linked to progeria impairs its phosphorylation at serine 22 during interphase. Moiseeva O, Lopes-Paciencia S, Huot G, Lessard F, Ferbeyre G. Aging (Albany NY). 2016 Feb;8(2):366-81. 10.18632/aging.100903 PubMed 26922519
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