pAAV-CAG-Peredox
(Plasmid #73807)

• Purpose: Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)
• Depositing Lab: Gary Yellen
• Publication: Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Backbone

• Vector backbone: AAV-Flex
• Modifications to backbone: Made cre-independent by passing through HEK293 cre-expressing cells.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Peredox
• Species: Synthetic

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid expresses only Peredox, with no mCherry or mCitrine to use for ratio measurements. It is best used for fluorescence lifetime imaging (FLIM).