pRSetB-Peredox
(Plasmid #73806)

• Purpose: Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag
• Depositing Lab: Gary Yellen
• Publication: Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Backbone

• Vector backbone: pRSetB
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Peredox
• Species: Synthetic
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag to facilitate purification (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid expresses only Peredox, with no mCherry or mCitrine to use for ratio measurements. It is best used for fluorescence lifetime measurements.