Nabi2.30
(Plasmid
#73798)
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PurposeNabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUB2.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7610
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNabi2.30
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nabi2.30 was a gift from Bradley Baker (Addgene plasmid # 73798 ; http://n2t.net/addgene:73798 ; RRID:Addgene_73798) -
For your References section:
Developing Fast Fluorescent Protein Voltage Sensors by Optimizing FRET Interactions. Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ. PLoS One. 2015 Nov 20;10(11):e0141585. doi: 10.1371/journal.pone.0141585. eCollection 2015. 10.1371/journal.pone.0141585 PubMed 26587834
