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pME pHluorin2
(Plasmid #73794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73794 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 73794-D50 50 μg of DNA in Tris buffer $495
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DNA 73794-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PCR8
  • Total vector size (bp) 3537
  • Vector type
    middle entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ratiometric pHluorin2
  • Species
    Synthetic

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Matthew Mahon

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ratiometric pHluorin2 was originally published in the following paper:

Mahon, M. (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein. Advances in Bioscience and Biotechnology, 2, 132-137. doi: 10.4236/abb.2011.23021.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME pHluorin2 was a gift from David Raible (Addgene plasmid # 73794 ; http://n2t.net/addgene:73794 ; RRID:Addgene_73794)

  • For your References section:

    The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity and function. Stawicki TM, Owens KN, Linbo T, Reinhart KE, Rubel EW, Raible DW. Dis Model Mech. 2014 Jul;7(7):847-56. doi: 10.1242/dmm.016576. 10.1242/dmm.016576 PubMed 24973752
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