pME eos
(Plasmid
#73792)
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PurposePhotoconvertible eos in a middle entry clone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
- Total vector size (bp) 3233
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Vector typemiddle entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEOS
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Insert Size (bp)681
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Eos is originally cited in the following paper: Wiedenmann J., Ivanchenko S., Oswald F., Schmitt F., Röcker C., Salih A., Spindler K.-D., Nienhaus G. U. (2004). EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA 101, 15905–15910.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME eos was a gift from David Raible (Addgene plasmid # 73792 ; http://n2t.net/addgene:73792 ; RRID:Addgene_73792) -
For your References section:
The metalloproteinase inhibitor Reck is essential for zebrafish DRG development. Prendergast A, Linbo TH, Swarts T, Ungos JM, McGraw HF, Krispin S, Weinstein BM, Raible DW. Development. 2012 Mar;139(6):1141-52. doi: 10.1242/dev.072439. Epub 2012 Feb 1. 10.1242/dev.072439 PubMed 22296847