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pET151-GFP-PP-RPN2 (932-953)
(Plasmid #73745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151-D-TOPO
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6682
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPN2
  • Alt name
    S1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    45
  • Mutation
    Construct possesses only residues 932-953 of RPN2
  • GenBank ID
    NM_001191037.1
  • Entrez Gene
    RPN2 (a.k.a. RIBIIR, RPN-II, RPNII, SWP1)
  • Promoter T7
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • 6x His (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Residues 932 and 933 of RPN2 are GP, which are also the C-terminal residues of the PreScission Protease recognition sequence that directly precedes RPN2 in our construct. For this reason, the inserted sequence of RPN2 corresponds to residues 934-953, which will result in the expression of the RPN2 932-953 peptide sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-GFP-PP-RPN2 (932-953) was a gift from Chris Hill (Addgene plasmid # 73745 ; http://n2t.net/addgene:73745 ; RRID:Addgene_73745)

  • For your References section:

    Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism. VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP. J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 10.1074/jbc.M117.785287. 10.1074/jbc.M117.785287 PubMed 28442575
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