pET151-GST-PP-RPN13 (19-132)
(Plasmid #73742)

• Purpose: Expression of human RPN13 PRU domain in E.coli
• Depositing Lab: Chris Hill
• Publication: VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 10.1074/jbc.M117.785287.

Backbone

• Vector backbone: pET151-D-TOPO
• Backbone size w/o insert (bp): 5800
• Total vector size (bp): 6829
• Modifications to backbone: N-terminal His-tag removed
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPN13
• Alt name: ADRM1
• Species: H. sapiens (human)
• Insert Size (bp): 345
• Mutation: PRU domain only (residues 19-132)
• GenBank ID: NM_175573
• Entrez Gene: ADRM1 (a.k.a. ARM-1, ARM1, GP110, PSMD16)
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry