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Addgene

HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector
(Plasmid #73706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIPZ
  • Backbone manufacturer
    GE Dharmacon
  • Backbone size w/o insert (bp) 12300
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RIPK3
  • Species
    M. musculus (mouse)
  • Mutation
    448-AAAA-451 and D143N
  • Entrez Gene
    Ripk3 (a.k.a. 2610528K09Rik, Rip3)
  • Promoter CMV/tet
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer acggtgggaggcctatataagc
  • 3′ sequencing primer tcgctgcgcccttcgtctgacg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector was a gift from Francis Chan (Addgene plasmid # 73706 ; http://n2t.net/addgene:73706 ; RRID:Addgene_73706)
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