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Addgene

pLOT-syb-sp-YFP1-10
(Plasmid #73700)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73700 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLOT
  • Backbone size w/o insert (bp) 9783
  • Total vector size (bp) 10200
  • Vector type
    Insect Expression ; LexAop reporter vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Neuronal-synaptobrevin:spYFP1–10 chimera
  • Species
    D. melanogaster (fly)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer HSP70
  • 3′ sequencing primer SV40
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The spYFP1–10 portion was created by mutagenizing spGFP1–10 which was amplified from ace-4 CD4-2 spGFP1–10 (gift of C. Bargmann)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOT-syb-sp-YFP1-10 was a gift from Marco Gallio (Addgene plasmid # 73700 ; http://n2t.net/addgene:73700 ; RRID:Addgene_73700)
  • For your References section:

    Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation. Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z, Lee CH, Gallio M. Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024. 10.1038/ncomms10024 PubMed 26635273