pLOT-syb-sp-YFP1-10
(Plasmid #73700)

• Purpose: Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP
• Depositing Lab: Marco Gallio
• Publication: Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.

Backbone

• Vector backbone: pLOT
• Backbone size w/o insert (bp): 9783
• Total vector size (bp): 10200
• Vector type: Insect Expression ; LexAop reporter vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Neuronal-synaptobrevin:spYFP1–10 chimera
• Species: D. melanogaster (fly)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: HSP70
• 3′ sequencing primer: SV40

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The spYFP1–10 portion was created by mutagenizing spGFP1–10 which was amplified from ace-4 CD4-2 spGFP1–10 (gift of C. Bargmann)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry