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Addgene

pMLS236
(Plasmid #73684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Worm Expression, Cre/Lox, CRISPR
  • Selectable markers
    Cbr-unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP + Cbr-unc-119
  • Species
    Synthetic
  • Tag / Fusion Protein
    • GFP

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The PU6::sgRNA cassette was derived from the PU6::sgRNA cassette in Addgene plasmid #46169 from John Calarco.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also encodes PU6::sgRNA cassette; The sgRNA targeting sequence can be sequenced with oMLS471: 5'-TCCAAGAACTCGTACAAAAATGCTC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMLS236 was a gift from Erik Jorgensen (Addgene plasmid # 73684 ; http://n2t.net/addgene:73684 ; RRID:Addgene_73684)

  • For your References section:

    SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. 10.1534/genetics.115.184275 PubMed 26837755
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