pMLS234
(Plasmid #73682)

• Purpose: SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker
• Depositing Lab: Erik Jorgensen
• Publication: Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.184275.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Thermo Fisher
• Vector type: Worm Expression, Cre/Lox, CRISPR
• Selectable markers: Cbr-unc-119

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP + Cbr-unc-119
• Species: C. elegans (nematode); C. briggsae
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 (-21) Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pMLS234 Genbank
• A portion of this plasmid was derived from a plasmid made by: Also encodes PU6::sgRNA cassette; The sgRNA targeting sequence can be sequenced with oMLS471: 5'-TCCAAGAACTCGTACAAAAATGCTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also encodes PU6::sgRNA cassette; The sgRNA targeting sequence can be sequenced with oMLS471: 5'-TCCAAGAACTCGTACAAAAATGCTC