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pSIREN-RetroQ-BPTF-sh3
(Plasmid #73667)

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Item Catalog # Description Quantity Price (USD)
Plasmid 73667 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pSIREN RetroQ
  • Backbone manufacturer
    Clonetech
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA #3 to BPTF
  • gRNA/shRNA sequence
    Bptf
  • Species
    H. sapiens (human), M. musculus (mouse)
  • GenBank ID
    NM_004459.6 NM_176850.2
  • Entrez Gene
    BPTF (a.k.a. FAC1, FALZ, NEDDFL, NURF301)
  • Entrez Gene
    Bptf (a.k.a. 9430093H17Rik, Fa, Falz)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer U6 Promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIREN-RetroQ-BPTF-sh3 was a gift from Joe Landry (Addgene plasmid # 73667 ; http://n2t.net/addgene:73667 ; RRID:Addgene_73667)

  • For your References section:

    BPTF inhibits NK cell activity and the abundance of natural cytotoxicity receptor co-ligands. Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic B, Dumur CI, Landry JW. Oncotarget. 2017 May 12;8(38):64344-64357. doi: 10.18632/oncotarget.17834. eCollection 2017 Sep 8. 10.18632/oncotarget.17834 PubMed 28969075
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