pOZ-FH-C Bach1 S990A
(Plasmid
#73647)
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PurposeRetroviral expression of Bach1 S990A mutant (BRCA binding mutant).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOZ-FH-C
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Backbone manufacturerNakatani and Ogryzko, 2003
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBach1
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Alt nameBTB and CNC homology 1, basic leucine zipper transcription factor 1
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SpeciesH. sapiens (human)
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MutationS990A (BRCA binding mutant)
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Entrez GeneBACH1 (a.k.a. BACH-1, BTBD24)
- Promoter unknown
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Tags
/ Fusion Proteins
- His (C terminal on backbone)
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBMN-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOZ-FH-C Bach1 S990A was a gift from Sharon Cantor (Addgene plasmid # 73647 ; http://n2t.net/addgene:73647 ; RRID:Addgene_73647)