pCDNA3.1 Bach1 K141A/K142A
(Plasmid
#73642)
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PurposeMammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBach1
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Alt nameBTB and CNC homology 1, basic leucine zipper transcription factor 1
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SpeciesH. sapiens (human)
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MutationK141A/K142A ( mlh1 binding mutant)
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Entrez GeneBACH1 (a.k.a. BACH-1, BTBD24)
- Promoter CMV
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Tags
/ Fusion Proteins
- Myc (C terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1 Bach1 K141A/K142A was a gift from Sharon Cantor (Addgene plasmid # 73642 ; http://n2t.net/addgene:73642 ; RRID:Addgene_73642)