pET22-HT/prsA
(Plasmid #73624)

• Purpose: Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag
• Depositing Labs: T. J. Kappock, James Williamson
• Publication: Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.

Backbone

• Vector backbone: pET22b
• Total vector size (bp): 6400
• Modifications to backbone: pET22b-HT adds a 17-residue sequence including a N-terminal His6 tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ribose-phosphate diphosphokinase
• Alt name: prsA
• Alt name: JW1198
• Alt name: b1207
• Species: Escherichia coli str. K-12 substr. MG1655
• GenBank ID: NP_415725.1 AAC74291.1
• Entrez Gene: prs (a.k.a. b1207, ECK1195, JW1198, dnaR, prsA)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry