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pET22-HT/purC
(Plasmid #73623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Total vector size (bp) 6132
  • Modifications to backbone
    pET22b-HT adds a 17-residue sequence including a N-terminal His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phosphoribosylaminoimidazolesuccinocarboxamide synthase
  • Alt name
    purC
  • Alt name
    b2476
  • Alt name
    JW2461
  • Species
    Escherichia coli str. K-12 substr. MG1655
  • Mutation
    Contains multiple silent mutations (E179, G185, G190, G197, T205, and E223).
  • GenBank ID
    NP_416971.1 AAC75529.1
  • Entrez Gene
    purC (a.k.a. b2476, ECK2472, JW2461, ade)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from E. coli K12 MRE6000

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22-HT/purC was a gift from T. J. Kappock & James Williamson (Addgene plasmid # 73623 ; http://n2t.net/addgene:73623 ; RRID:Addgene_73623)

  • For your References section:

    Pathway engineered enzymatic de novo purine nucleotide synthesis. Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR. ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p. 10.1021/cb800066p PubMed 18707057
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