pLex-FLAG-FASTKD4 mutant delta 226
(Plasmid
#73621)
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PurposeExpression vector containing FASTKD4 mutant delta 226
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLex-FLAG
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFASTKD4
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Alt nameTBRG4
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SpeciesH. sapiens (human)
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MutationY616A, L617A, K618A, K620A
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Entrez GeneTBRG4 (a.k.a. CPR2, FASTKD4)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLex-FLAG-FASTKD4 mutant delta 226 was a gift from Vamsi Mootha (Addgene plasmid # 73621 ; http://n2t.net/addgene:73621 ; RRID:Addgene_73621) -
For your References section:
Functional genomic analysis of human mitochondrial RNA processing. Wolf AR, Mootha VK. Cell Rep. 2014 May 8;7(3):918-31. doi: 10.1016/j.celrep.2014.03.035. Epub 2014 Apr 18. 10.1016/j.celrep.2014.03.035 PubMed 24746820