pET15b/bPanK
(Plasmid #73619)

• Purpose: Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)
• Depositing Labs: Suzanne Jackowski, T. J. Kappock
• Publication: Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Backbone

• Vector backbone: pET15b
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 6672
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pantothenate kinase
• Alt name: CoaA
• Alt name: panK
• Species: Escherichia coli
• Mutation: relative to MG1655 sequence, changes glutamate-5 to aspartate
• GenBank ID: WP_001305325.1|
• Entrez Gene: coaA (a.k.a. b3974, ECK3966, JW3942, panK, rts, ts-9)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Amplified gene from pWS7-13-2 [described in WJ Song & S Jackowski (1992) J. Bacteriol. 174: 6411 (PMID 1328157)]. Cloning described as blunt ligation of blunted panK fragment (AcyI/PstI) into blunted BamHI cut pET15b. The "restriction sites" described as used for cloning (NdeI-BamHI) are unique in the plasmid.