pJK370
(Plasmid #73595)

• Purpose: Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
• Depositing Lab: T. J. Kappock
• Publication: Additional fatty acid desaturase and acetylenase constructs (unpublished)

Backbone

• Vector backbone: pESC-URA
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 6631
• Total vector size (bp): 8886
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: delta-12 fatty acid acetylenase
• Alt name: vFAD2
• Species: Crepis alpina
• GenBank ID: ABC00769.1
• Promoter: GAL10
• Tag / Fusion Protein:
  • FLAG tag (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: GAL10 forward primer
• 3′ sequencing primer: GAL10 reverse primer

Gene/Insert 2

• Gene/Insert name: delta-12 fatty acid desaturase (form 2)
• Alt name: FAD2-02
• Alt name: Fad2
• Species: Crepis alpina
• Mutation: Silent mutation in Arg299 codon.
• GenBank ID: DQ289486
• Promoter: GAL1
• Tag / Fusion Protein:
  • c-myc tag (C terminal on backbone)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: GAL1 forward primer
• 3′ sequencing primer: GAL1 reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry