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Addgene

pJK369
(Plasmid #73594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESC-URA
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 6631
  • Total vector size (bp) 7764
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta-12 fatty acid acetylenase
  • Alt name
    vFAD2
  • Species
    Crepis alpina
  • GenBank ID
    ABC00769.1
  • Promoter GAL10
  • Tag / Fusion Protein
    • FLAG tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer GAL10 forward primer
  • 3′ sequencing primer GAL10 reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Empty MCS2 site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK369 was a gift from T. J. Kappock (Addgene plasmid # 73594 ; http://n2t.net/addgene:73594 ; RRID:Addgene_73594)
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