hTRAFD1-pCMV14-3xFlag
(Plasmid #73587)

• Purpose: Express human TRAFD1 (FLN29) in mammalian cells
• Depositing Lab: Paul Odgren
• Publication: Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. doi: 10.1371/journal.pone.0127537. eCollection 2015.

Backbone

• Vector backbone: pCMV-14-3xFlag
• Backbone manufacturer: Sigma Aldrich
• Backbone size w/o insert (bp): 6270
• Total vector size (bp): 8019
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRAFD1
• Alt name: FLN29
• Species: H. sapiens (human)
• Insert Size (bp): 1749
• Mutation: The original vector hTRAFD1-mCMV14-Flag was a gift from Dr. Akihiko Yoshimura, Keio University, Tokyo, Japan. We removed the stop codon from TRAFD1 (prior to Flag tag) so we could pull down Trafd1 using Flag beads. Flag doesn't have start codon
• Entrez Gene: TRAFD1 (a.k.a. FLN29)
• Tag / Fusion Protein:
  • 3xFlag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV fwd primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry