pLenti-CMV-MCS-GFP-SV-puro
(Plasmid #73582)

• Purpose: (Empty Backbone) Third generation lentiviral vector expressing GFP and puromycin
• Depositing Lab: Paul Odgren
• Publication: Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi: 10.1371/journal.pone.0128275. eCollection 2015.

Backbone

• Vector backbone: pLenti-CMV-MCs-SV-puro
• Backbone manufacturer: gift from Dr. Hong Zhang, University of Massachusetts Medical School
• Backbone size (bp): 8829
• Modifications to backbone: GFP insert
• Vector type: Lentiviral
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • GFP tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GAGCACCCAGTCCGCCCTG

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 73582-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here