C321.deltaA (Isaacs lab)
(Bacterial strain #73581)

• Purpose: Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene
• Depositing Lab: Farren Isaacs
• Publication: Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.

Backbone

• Vector backbone: NA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): This is a strain
• Growth instructions: Growth above 34 C is not recommended, since growth at elevated temperatures leads to leaky expression of toxic proteins from the lambda prophage.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: none
• Alt name: C321.ΔA.1
• Alt name: C321.deltaA.1

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli MG1655 ΔmutS:zeo.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA; all 321 UAG codons changed to UAA

This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. This is the exact strain used for protein expression in publication PMID:26571098.