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Addgene

MSCV-miRE-shRNA IFT88-PGK-neo-IRES-GFP
(Plasmid #73576)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    miR-E
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFT88
  • Alt name
    shRNA IFT88
  • gRNA/shRNA sequence
    TAG AAA TTG ATG AAG ATG ATA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ift88 (a.k.a. Tg737, Tg737Rpw, TgN737Rpw, Ttc10, flexo, fxo, orpk, polaris)
  • Promoter MSCV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-miRE-shRNA IFT88-PGK-neo-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 73576 ; http://n2t.net/addgene:73576 ; RRID:Addgene_73576)

  • For your References section:

    The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Vo BT, Wolf E, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz S, Murphy BL, Youn YH, Han YG, Eilers M, Roussel MF. Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 10.1016/j.ccell.2015.12.003. 10.1016/j.ccell.2015.12.003 PubMed 26766587
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