MSCV-mouse-MycNV419D-IRES-RFP
(Plasmid #73574)

• Purpose: Mammalian expression of mouse MycN V419D mutant
• Depositing Lab: Martine Roussel
• Publication: Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 10.1016/j.ccell.2015.12.003.

Backbone

• Vector backbone: pMSCV-IRES RFP
• Backbone size w/o insert (bp): 6573
• Total vector size (bp): 7873
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mycn
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: V419D
• Entrez Gene: Mycn (a.k.a. N-myc, Nmyc, Nmyc-1, Nmyc1, bHLHe37, c-nmyc)
• Promoter: MSCV
• Tag / Fusion Protein:
  • IRES-RFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pLXSN
• 3′ sequencing primer: pCDH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry