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Addgene

CMV-CAD
(Plasmid #73567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGCP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3980
  • Total vector size (bp) 4307
  • Modifications to backbone
    The EGFP gene was deleted and replaced with the CAD gene (STIM1 342-448).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAD (STIM1 342-448)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    327
  • GenBank ID
    NM_003156
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Promoter CMV Immediate Early

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGACGTCAATGGGAGTTTG
  • 3′ sequencing primer ACATTGATGAGTTTGGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CAD corresponds to amino acids 342-448 of human STIM1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-CAD was a gift from Michael Cahalan (Addgene plasmid # 73567 ; http://n2t.net/addgene:73567 ; RRID:Addgene_73567)

  • For your References section:

    Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Dynes JL, Amcheslavsky A, Cahalan MD. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 10.1073/pnas.1523410113. Epub 2015 Dec 28. 10.1073/pnas.1523410113 PubMed 26712003
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