mCherry-CAD
(Plasmid #73566)

• Purpose: Soluble red fluorescent activator of Orai1 channels
• Depositing Lab: Michael Cahalan
• Publication: Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 10.1073/pnas.1523410113. Epub 2015 Dec 28.

Backbone

• Vector backbone: pmChery-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3954
• Total vector size (bp): 5046
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-CAD (STIM1 342-448)
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1092
• GenBank ID: NM_003156
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV Immediate Early
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • 6x His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: TGACGTCAATGGGAGTTTG
• 3′ sequencing primer: ACATTGATGAGTTTGGAC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CAD corresponds to amino acids 342-448 of human STIM1.