shCDK4/6(2)
(Plasmid
#73555)
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Purposevector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMLPX
- Total vector size (bp) 8000
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Modifications to backbone+GFP
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshRNA against CDK4 + shRNA against CDK6
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gRNA/shRNA sequenceCDK4:GGCACTTACACCCGTGGTTGTT, CDK6: ACCGAGTAGTGCATCGCGATCT
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SpeciesH. sapiens (human)
- Promoter LTR
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CCCTTGAACCTCCTCGTTCGACC
- 3′ sequencing primer CTTCGCGCCACCTTCTACTCCT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
shCDK4/6(2) was a gift from Gerardo Ferbeyre (Addgene plasmid # 73555 ; http://n2t.net/addgene:73555 ; RRID:Addgene_73555) -
For your References section:
A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from PML-induced Senescence. Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, Bourdeau V, Ferbeyre G. Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29. 10.1158/0008-5472.CAN-15-2347 PubMed 27206849