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Addgene

pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3
(Plasmid #73542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
  • Backbone manufacturer
    Kosuke Yusa (Addgene plasmid # 50946)
  • Backbone size w/o insert (bp) 8102
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LINE-1
  • gRNA/shRNA sequence
    CCAGAGAACCTGACAGCTTC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    20
  • Promoter hU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer tgtaaacacaaagatattagtacaa
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3 was a gift from Constance Ciaudo (Addgene plasmid # 73542 ; http://n2t.net/addgene:73542 ; RRID:Addgene_73542)

  • For your References section:

    Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition. Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C. EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458. 10.15252/embr.202154458 PubMed 35856394
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