pEGFP-ninein C-ter
(Plasmid #73523)

• Purpose: Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus
• Depositing Lab: Michel Bornens
• Publication: Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75. Epub 2005 Mar 22.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ninein C-ter (residues 1874-2113)
• Species: M. musculus (mouse)
• Entrez Gene: Nin (a.k.a. 3110068G20Rik, AI385615, AU024711, mKIAA1565)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: EGFP 3' Fw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned by Nathalie DELGEHYR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry