pEGFP-ninein N-ter
(Plasmid
#73520)
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PurposeEncodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminus
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameninein N-ter (residues 1-373)
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SpeciesM. musculus (mouse)
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Entrez GeneNin (a.k.a. 3110068G20Rik, AI385615, AU024711, mKIAA1565)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP 3' Fw (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by Nathalie DELGEHYR
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-ninein N-ter was a gift from Michel Bornens (Addgene plasmid # 73520 ; http://n2t.net/addgene:73520 ; RRID:Addgene_73520) -
For your References section:
Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. Delgehyr N, Sillibourne J, Bornens M. J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75. Epub 2005 Mar 22. 10.1242/jcs.02302 PubMed 15784680