pJK323
(Plasmid
#73495)
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PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC118
- Backbone size w/o insert (bp) 3162
- Total vector size (bp) 4723
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namehypothetical protein
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Alt nameorfX
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Alt nameAZ09_02685
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SpeciesAcetobacter aceti 1023
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GenBank IDAAZ04482.1 KDE21028.1
Gene/Insert 2
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Gene/Insert nameN5-carboxyaminoimidazole ribonucleotide synthetase
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Alt namepurK
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Alt nameAZ09_02690
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SpeciesAcetobacter aceti 1023
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GenBank IDAAZ04484.1 KDE21030.1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Last 40 nucleotides of purE gene kept.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK323 was a gift from T. J. Kappock (Addgene plasmid # 73495 ; http://n2t.net/addgene:73495 ; RRID:Addgene_73495)