366 pTol2-CherryDest
(Plasmid
#73487)
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Purposepromoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-attR1/attR2
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SpeciesSynthetic
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MutationH30R (please see depositor comments below)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
366 pTol2-CherryDest was a gift from Nathan Lawson (Addgene plasmid # 73487 ; http://n2t.net/addgene:73487 ; RRID:Addgene_73487) -
For your References section:
Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311
