251 pTol2Dest
(Plasmid #73484)

• Purpose: promoterless Tol2 vector with attR1/attR2 cassette and late SV40 polyadenylation signal
• Depositing Lab: Nathan Lawson
• Publication: Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Backbone

• Vector backbone: pTol2005b

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: attR1/attR2 cassette
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: PspOMI (not destroyed)
• 5′ sequencing primer: M13-FP
• 3′ sequencing primer: M13R-pUC

Resource Information

• Supplemental Documents:
  • pTol2Dest GB map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry