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Addgene

DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)
(Plasmid #73478)

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 73478 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV Ef1a DIO
  • Backbone size w/o insert (bp) 8382
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can also be grown in Stbl2 or Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H2B-3XHA-P2A-N2c(G)
  • Insert Size (bp)
    2272

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G) was a gift from Thomas Jessell (Addgene plasmid # 73478 ; http://n2t.net/addgene:73478 ; RRID:Addgene_73478)
  • For your References section:

    Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A. Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. 10.1016/j.neuron.2016.01.004 PubMed 26804990