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pZW1-FCS-circRNA
(Plasmid #73449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZW1
  • Backbone manufacturer
    Zefeng Wang's lab
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POLR2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2125
  • Mutation
    none
  • Entrez Gene
    POLR2A (a.k.a. NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZW1-FCS-circRNA was a gift from Ling-Ling Chen (Addgene plasmid # 73449 ; http://n2t.net/addgene:73449 ; RRID:Addgene_73449)

  • For your References section:

    Complementary sequence-mediated exon circularization. Zhang XO, Wang HB, Zhang Y, Lu X, Chen LL, Yang L. Cell. 2014 Sep 25;159(1):134-47. doi: 10.1016/j.cell.2014.09.001. Epub 2014 Sep 18. 10.1016/j.cell.2014.09.001 PubMed 25242744
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