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Addgene

mOC-STAMP_pcDNA3.1 D/V5-His-TOPO
(Plasmid #73448)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73448 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 D V5-His
  • Backbone size w/o insert (bp) 5052
  • Total vector size (bp) 7008
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ocstamp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1956
  • Mutation
    N162D
  • GenBank ID
    NM_029021.1
  • Entrez Gene
    Ocstamp (a.k.a. 4833422F24Rik, OC-STAMP)
  • Tag / Fusion Protein
    • V5-6xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The OC-stamp insert contains an N162D mutation. This mutation affects protein glycosylation but is not known to disrupt protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mOC-STAMP_pcDNA3.1 D/V5-His-TOPO was a gift from Paul Odgren (Addgene plasmid # 73448 ; http://n2t.net/addgene:73448 ; RRID:Addgene_73448)
  • For your References section:

    Osteoclast stimulatory transmembrane protein (OC-STAMP), a novel protein induced by RANKL that promotes osteoclast differentiation. Yang M, Birnbaum MJ, MacKay CA, Mason-Savas A, Thompson B, Odgren PR. J Cell Physiol. 2008 May;215(2):497-505. 10.1002/jcp.21331 PubMed 18064667