mOC-STAMP_pcDNA3.1 D/V5-His-TOPO
(Plasmid
#73448)
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PurposeExpress mouse OC-STAMP in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 D V5-His
- Backbone size w/o insert (bp) 5052
- Total vector size (bp) 7008
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOcstamp
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SpeciesM. musculus (mouse)
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Insert Size (bp)1956
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MutationN162D
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GenBank IDNM_029021.1
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Entrez GeneOcstamp (a.k.a. 4833422F24Rik, OC-STAMP)
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Tag
/ Fusion Protein
- V5-6xHis (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV fwd primer
- 3′ sequencing primer BGH rev primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The OC-stamp insert contains an N162D mutation. This mutation affects protein glycosylation but is not known to disrupt protein function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO was a gift from Paul Odgren (Addgene plasmid # 73448 ; http://n2t.net/addgene:73448 ; RRID:Addgene_73448) -
For your References section:
Osteoclast stimulatory transmembrane protein (OC-STAMP), a novel protein induced by RANKL that promotes osteoclast differentiation. Yang M, Birnbaum MJ, MacKay CA, Mason-Savas A, Thompson B, Odgren PR. J Cell Physiol. 2008 May;215(2):497-505. 10.1002/jcp.21331 PubMed 18064667