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Purpose(Empty Backbone) expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73447 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYD1
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Vector typeYeast Expression
- Promoter GAL1
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Selectable markersTRP1
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Tags
/ Fusion Proteins
- AGA2 (N terminal on backbone)
- T7 peptide (N terminal on backbone)
- Xpress tag (N terminal on backbone)
- V5 tag (C terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYD1 was a gift from Dane Wittrup (Addgene plasmid # 73447 ; http://n2t.net/addgene:73447 ; RRID:Addgene_73447) -
For your References section:
Isolation of anti-T cell receptor scFv mutants by yeast surface display. Kieke MC, Cho BK, Boder ET, Kranz DM, Wittrup KD. Protein Eng. 1997 Nov;10(11):1303-10. PubMed 9514119
Map uploaded by the depositor.
