pCRT7 NT Topo arabinose
(Plasmid
#73446)
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Purpose(Empty Backbone) Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRT7/NT-TOPO
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Vector typeBacterial Expression
- Promoter pBad
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Tag
/ Fusion Protein
- GST (N-terminal), 6xHis (C-terminal)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRT7 NT Topo arabinose was a gift from Jesse Rinehart (Addgene plasmid # 73446 ; http://n2t.net/addgene:73446 ; RRID:Addgene_73446)
