pETM6-G6-vioABE-4A6-vioC
(Plasmid #73439)

• Purpose: Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.
• Depositing Lab: Mattheos Koffas
• Publication: Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw231.

Backbone

• Vector backbone: pETM6
• Backbone manufacturer: Koffas Lab, Addgene Plasmid #49795
• Backbone size w/o insert (bp): 5147
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PG6-vioABE-P4A6-vioC
• Species: Pseudoalteromonas luteoviolacea strain B
• Promoter: PG6 and P4A6 (orthogonal T7-lac variants)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBRrevBam

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Coexpression with G6 CRISPR repressor shuts off all colored metabolite production. Coexpression with 4A6 CRISPR repressor switches carbon flux from deoxyviolacein production (red-violet culture) to prodeoxyviolacein (green culture).