pETM6-3A2-mCherry
(Plasmid #73425)

• Purpose: Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
• Depositing Lab: Mattheos Koffas
• Publication: Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw231.

Backbone

• Vector backbone: pETM6
• Backbone manufacturer: Koffas Lab, Addgene Plasmid #49795
• Backbone size w/o insert (bp): 5147
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Promoter 3A2 (orthogonal T7-lac variant)
• Species: Synthetic
• Promoter: P3A2 (orthogonal T7-lac variant)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBRrevBam
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.