PKC-delta-IRES-hrGFP
(Plasmid
#73414)
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PurposeMammalian expression of mouse PKC-delta and hrGFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES-hr-GFP
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePKC-delta
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Alt nameprotein kinase C, delta
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SpeciesM. musculus (mouse)
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GenBank IDNP_001397611.1
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Entrez GenePrkcd (a.k.a. D14Ertd420e, PKC[d], PKCdelta, Pkcd)
- Promoter CMV
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Tags
/ Fusion Proteins
- 3xFLAG (C terminal on backbone)
- IRES-hrGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKC-delta-IRES-hrGFP was a gift from Raymond Swanson (Addgene plasmid # 73414 ; http://n2t.net/addgene:73414 ; RRID:Addgene_73414) -
For your References section:
Phosphoinositide 3-kinase couples NMDA receptors to superoxide release in excitotoxic neuronal death. Brennan-Minnella AM, Shen Y, El-Benna J, Swanson RA. Cell Death Dis. 2013 Apr 4;4:e580. doi: 10.1038/cddis.2013.111. 10.1038/cddis.2013.111 PubMed 23559014