pET28b-LanCL2 (6xHis-LanCL2)
(Plasmid
#73407)
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PurposeExpresses His tagged LanCL2
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLanCL2
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SpeciesH. sapiens (human)
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Entrez GeneLANCL2 (a.k.a. GPR69B, TASP)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-LanCL2 (6xHis-LanCL2) was a gift from Jie Chen (Addgene plasmid # 73407 ; http://n2t.net/addgene:73407 ; RRID:Addgene_73407) -
For your References section:
Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Zeng M, van der Donk WA, Chen J. Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 10.1091/mbc.E14-01-0004. Epub 2014 Oct 1. 10.1091/mbc.E14-01-0004 PubMed 25273559