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PKM-IRES-hrGFP
(Plasmid #73405)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-hr-GFP
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKM
  • Alt name
    protein kinase M
  • Alt name
    truncated, constitutively active form of PKC zeta
  • Species
    M. musculus (mouse)
  • Mutation
    truncated, constitutively active form
  • GenBank ID
    NM_001039079
  • Entrez Gene
    Prkcz (a.k.a. Pkcz, aPKCzeta, nPKC-zeta, zetaPKC)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (C terminal on backbone)
    • pIRES-hrGFP-2a (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PKM (Genebank accession NM_001039079) was amplified with primers 5′-tgcgaattcaatggattctgtcatgccttcc-3′ and 5′-attgtcgactcacacggactcctcagcag-3′ from adult mouse brain cDNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKM-IRES-hrGFP was a gift from Raymond Swanson (Addgene plasmid # 73405 ; http://n2t.net/addgene:73405 ; RRID:Addgene_73405)

  • For your References section:

    Phosphoinositide 3-kinase couples NMDA receptors to superoxide release in excitotoxic neuronal death. Brennan-Minnella AM, Shen Y, El-Benna J, Swanson RA. Cell Death Dis. 2013 Apr 4;4:e580. doi: 10.1038/cddis.2013.111. 10.1038/cddis.2013.111 PubMed 23559014
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