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pETM6-At4CL-PhCHS-CmCHI
(Plasmid #73393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM6
  • Backbone manufacturer
    Koffas Lab, Addgene Plasmid #49795
  • Backbone size w/o insert (bp) 5203
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    At4CL
  • Species
    A. thaliana (mustard weed)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    PhCHS
  • Species
    P. hybrida
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    CmCHI
  • Species
    C. maxima
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6-At4CL-PhCHS-CmCHI was a gift from Mattheos Koffas (Addgene plasmid # 73393 ; http://n2t.net/addgene:73393 ; RRID:Addgene_73393)

  • For your References section:

    Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance DM, Hahn J, Koffas MA. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. 10.1016/j.ymben.2016.01.006 PubMed 26860871
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