pRK5-HA-YFP-PRAS40
(Plasmid
#73389)
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PurposeExpresses HA and YFP tagged PRAS40
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAKT1 Substrate 1 (Proline-Rich)
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SpeciesH. sapiens (human)
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Entrez GeneAKT1S1 (a.k.a. Lobe, PRAS40)
- Promoter CMV
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- YFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-HA-YFP-PRAS40 was a gift from Jie Chen & Taekjip Ha (Addgene plasmid # 73389 ; http://n2t.net/addgene:73389 ; RRID:Addgene_73389) -
For your References section:
Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown. Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 10.1073/pnas.1419425111. Epub 2014 Dec 1. 10.1073/pnas.1419425111 PubMed 25453101