pCDNA-mSin1(1.1)-HA
(Plasmid #73388)

• Purpose: Expresses mSin-HA
• Depositing Labs: Jie Chen, Taekjip Ha
• Publication: Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 10.1073/pnas.1419425111. Epub 2014 Dec 1.

Backbone

• Vector backbone: pCDNA3
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mSin1 transcript variant protein isoform 1
• Alt name: MAPKAP1
• Species: H. sapiens (human)
• Entrez Gene: MAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry