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Addgene

pRK5-HA-mCherry-raptor
(Plasmid #73386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Regulatory Associated Protein Of MTOR, Complex 1
  • Alt name
    raptor
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPTOR (a.k.a. KOG1, Mip1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-mCherry-raptor was a gift from Jie Chen & Taekjip Ha (Addgene plasmid # 73386 ; http://n2t.net/addgene:73386 ; RRID:Addgene_73386)

  • For your References section:

    Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown. Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 10.1073/pnas.1419425111. Epub 2014 Dec 1. 10.1073/pnas.1419425111 PubMed 25453101
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