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PurposeExpresses YFP tagged mTOR
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73384 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepeYFP–C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemTOR
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SpeciesH. sapiens (human)
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
- Promoter CMV
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
peYFP-C1-mTOR was a gift from Jie Chen & Taekjip Ha (Addgene plasmid # 73384 ; http://n2t.net/addgene:73384 ; RRID:Addgene_73384) -
For your References section:
Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown. Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 10.1073/pnas.1419425111. Epub 2014 Dec 1. 10.1073/pnas.1419425111 PubMed 25453101
